LOCUS       pLV-[hsa-miR-302b_302c_302a_302d_367]   9828 bp    DNA     circular     12-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7749..8609
                     /label=Amp(R)
     promoter        8810..9350
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6354..6588
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(7163..7618)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6660..6790
                     /label=SV40 pA
     misc_feature    6791..7004
                     /label=SV40 ori
     misc_feature    5675..6271
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..4293
                     /label=intron
     CDS             4340..5665
                     /label=rPuro (RFP Puro)
     misc_RNA        3105..4088
                     /label=hsa-mir-302b_302c_302a_302d_367 precursor
BASE COUNT     2349 a      2485 c      2585 g      2409 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagtgcctt ttacccttct 
     3121 ggaggagaac acgaatcttt gggaactagt tcaggaaggt aaaaaaattt ttttcttcta 
     3181 aagttatgcc attttgtttt ctttctcctc agctctaaat actctgaagt ccaaagaagt 
     3241 tgtatgttgg gtgggctccc ttcaacttta acatggaagt gctttctgtg actttaaaag 
     3301 taagtgcttc catgttttag taggagtgaa tccaatttac ttctccaaaa tagaacacgc 
     3361 taacctcatt tgaagggatc ccctttgctt taacatgggg gtacctgctg tgtgaaacaa 
     3421 aagtaagtgc ttccatgttt cagtggaggt gtctccaagc cagcacacct tttgttacaa 
     3481 aatttttttg ttattgtgtt ttaaggttac taagcttgtt acaggttaaa ggattctaac 
     3541 tttttccaag actgggctcc ccaccactta aacgtggatg tacttgcttt gaaactaaag 
     3601 aagtaagtgc ttccatgttt tggtgatggt aagtcttcct tttacatttt tattattttt 
     3661 ttagaaaata actttattgt attgaccgca gctcatatat ttaagcttta ttttgtattt 
     3721 ttacatctgt taaggggccc cctctacttt aacatggagg cacttgctgt gacatgacaa 
     3781 aaataagtgc ttccatgttt gagtgtggtg gttcctacct aatcagcaat tgcgttaacg 
     3841 cccacactgt gtgcagttct tggctacagg ccattactgt tgctaatatg caactctgtt 
     3901 gaatataaat tggaattgca ctttagcaat ggtgatggat tgttaagcca atgacagaat 
     3961 ttaaaccaca gacttacttt gatagcactc ttaatggtat aacttcttct cccattttat 
     4021 gtctctcttt atgttttttc ttatgtttcc tttttgtttt caagagagag ctatctttta 
     4081 gatctccact cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg 
     4141 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg 
     4201 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag 
     4261 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaaaactacc cctctagagt 
     4321 cgagctaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa 
     4381 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat 
     4441 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac 
     4501 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc 
     4561 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga 
     4621 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca 
     4681 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt 
     4741 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg 
     4801 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga 
     4861 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct 
     4921 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 
     4981 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 
     5041 gtacaaggac ccaccggtcg ccaccatgac cgagtacaag cccacggtgc gcctcgccac 
     5101 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 
     5161 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 
     5221 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 
     5281 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 
     5341 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct 
     5401 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 
     5461 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 
     5521 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 
     5581 gcggctcggc ttcaccgtca ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg 
     5641 catgacccgc aagcccggtg cctgagcggc cgcgtcgaca atcaacctct ggattacaaa 
     5701 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 
     5761 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 
     5821 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 
     5881 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 
     5941 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 
     6001 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 
     6061 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 
     6121 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 
     6181 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 
     6241 cggatctccc tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa 
     6301 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 
     6361 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt 
     6421 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 
     6481 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 
     6541 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 
     6601 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 
     6661 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     6721 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     6781 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc 
     6841 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 
     6901 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 
     6961 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta 
     7021 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 
     7081 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 
     7141 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 
     7201 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 
     7261 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 
     7321 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 
     7381 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 
     7441 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 
     7501 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 
     7561 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag 
     7621 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     7681 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     7741 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     7801 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     7861 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     7921 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     7981 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     8041 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     8101 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     8161 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     8221 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     8281 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     8341 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     8401 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     8461 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     8521 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     8581 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     8641 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     8701 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     8761 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     8821 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     8881 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 
     8941 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     9001 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     9061 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     9121 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     9181 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     9241 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     9301 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     9361 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     9421 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
     9481 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
     9541 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 
     9601 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 
     9661 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 
     9721 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 
     9781 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgc 
//
